The directed-site membrane transferring and visual screening method based on plate were used and the factors which affect bacterium growth enzyme expression and the success rate of membrane transferring were optimized to develop the high through-put screening method for thermo stable p-glucanase .

本研究 将定位膜 转印和肉眼可见的平板筛选策略相结合，通过对影响菌落生长、 酶的表达和转印成功率的各因素的优化，建立了高通量筛选耐高温β-葡聚糖酶的方法。