Since the state transition from coding to non-coding is absent the exons and the introns of untranslated regions are all non-coding sequences . The identification of splice sites embedded in UTR is more challenge .

非翻译区剪接位点的两侧不存在编码到非编码的状态转移，因为它的内含子和外显 子均是非编码的，所以对 非 翻译 区中的剪接位点进行识别较难。

F ⅶ gene mutations were analysed in the proband by DNA direct sequencing of PCR products of all exons exon-intron boundaries and the 3 ′ 5 ′ untranslated sequences .

用DNA直接测序法对患者FⅦ基因的全部外显子和其侧翼以及3′，5′ 非 翻译 区进行分析，寻找基因突变，对有突变的 序列反向测序证实；

Objective To obtain the untranslated region ( UTR ) sequences of hepatitis E virus ( HEV ) isolated from Morocco to find whether the UTR sequences can be used to distinguish HEV genotypes from that of HEV complete genome sequences .

目的检测戊型肝炎病毒（HEV）摩洛哥株 非 编码区（UTR） 序列，探讨HEV非编码区序列能否作为其基因型分型的依据。

Methods All exons exon-intron boundaries and the 3 ′ 5 ′ untranslated sequences of F ⅶ and tissue factor ( TF ) genes were amplified by PCR and sequenced directly .

方法用DNA直接 测序法对先证者FⅦ及组织因子（TF）基因的全部外显子及其侧翼5′和3′ 非 翻译 区进行 分析，寻找突变基因。