Suppression subtractive hybridization ( SSH ) cDNA plasmid libraries were constructed between gastrula embryos and tail bud embryos in gynogenetic gibel carp ( Carassius auratus gibelio ) .

构建了雌核发育银鲫原肠期胚胎和 尾 芽 期胚胎间的抑制性差减杂交cDNA质粒文库。

One hundred and ninety two and 960 PCR positive clones were respectively selected to perform dot blot tests ; 15 and 131 dot blot positive clones were obtained from SSH plasmid libraries in the blastula and tail bud stage embryos .

分别取囊胚期胚胎和 尾 芽期胚胎各192和960个PCR阳性克隆进行斑点杂交，得到15个囊胚期和131个 尾 芽 期的斑点杂交阳性克隆。

The G-PAGE was used to analyze the proteinase activity of Rana nigromaculata during early embryonic development . The results showed that : ① Before tail bud stage there was almost no proteinase activity ;

本文采用活性电泳方法，对黑斑蛙（Rananigromaculata）早期胚胎发育过程中蛋白酶活性进行了研究.结果如下： 尾 芽期以前未检测到蛋白酶活性；

Method This study is aimed at the technical treatment with chemical physical and hormone factors during tail bud period in the process of embryonic development .

方法本研究在中国林蛙胚胎发育的 尾 芽期，用化学、物理和激素因子进行技术处理。

739 and 816 PCR positive clones were respectively selected to perform dot blot and 72 dot blot positive clones and 98 dot blot positive clones were obtained from the SSH plasmid libraries specific for gastrula embryos and tail bud embryos .

对原肠期739个和 尾 芽 期816个PCR阳性克隆进行斑点杂交，得到72个原肠期和98个 尾 芽 期斑点杂交阳性克隆。

Conclusion : Protein pattern of dimensional electrophoresis of tail bud embryos raried obviously after laser irradiation and there were relationships between protein pattern and laser energy density .

结论：激光照射对 尾 芽胚双向电泳蛋白质谱有明显影响，蛋白质点的变化与激光能量密度相关。

Embryos at tail bud stage are more tolerant to vitrification solutions .

在胚胎发育过程中，牙鲆各期胚胎对玻璃化液的适应能力不同， 尾 芽期胚胎较其他时期耐受力强。

Studies on Biological Properties and Subretinal Space Transplantation of Optic Cup-derived Retinal Stem Cells from Rat Tail Bud Embryo

大鼠视 杯 视网膜干细胞生物学特性和移植的实验研究