Methods The last two abdomen segments of mosquitoes were cut down and treated with KOH alcohol and xylene . Then the samples were observed under Nikon light microscope after sealed with neutral balsam on slides .

方法自雌蚊腹部剪下腹节最后两节，经KOH浸泡过夜后，酒精 脱水 、 二甲苯 透明， 加拿大 树胶封片后显微镜下观察。