Thrombopoietic effect of recombinant human thrombopoietin gene transferred to mice mediated by electric pulse on normal and experimental thrombocytopenia mice

电脉冲介导的Tpo基因转移对正常及实验性血小板减少小鼠的 促 血小板 生成作用

Objective To study the distribution characteristics of a new thrombopoietic factor in vivo in mice .

目的探讨本所 研制的新型 升 血小板因子在小鼠体内分布情况， 明确其 是否 具有 靶 向性分布的特点。

Study on immunogenicity of a new thrombopoietic factor

新型 升 血小板因子的免疫原性实验研究

The in vivo thrombopoietic activity was studied by subcutaneous administration of rhIL-6 to Balb / C mice every day for 7 days and blood was taken from tail vein for platelet counting .

将rhIL-6和 ss-PEG-rhIL-6每天给Balb/C小鼠皮下注射 持续7天，每天尾静脉取血，通过计数血小板来观察rhIL-6和ss-PEG-rhIL-6 促 血小板 增殖作用。

No antibody was found in all mice by ELISA . Conclusion The new thrombopoietic factor has no immunogenicity in BALB / c mouse .

结论用新型 升 血小板因子免疫Balb/c小鼠，未发现 能够 刺激小鼠产生抗 新型 升 血小板因子抗体。

Experimental study on targeting distribution characteristic of a new thrombopoietic factor in mice

新型 升 血小板因子 静脉 注射在小鼠 体内的靶向性实验研究

Ss-PEG modification can enhance the half-life and stability of rhIL-6 . ss-PEG-rhIL-6 may be a potential candidate for thrombopoietic agent .

ss－PEG修饰可提高rhIL－6的半衰期和稳定性，ss－PEG－rhIL－6是一种很有潜力的 促 血小板 增殖药物。