Study on the Activity of Tryptophanase in Genetic Engineering Strains and Relationship between It and Expression

色 氨 酸 酶基因工程菌的活力及其与表达关系研究

Methods : Effects of three immobilized materials temperature pH monovalent canons and alcohol on the activity of tryptophanase of immobilized WW-11 were observed by enzyme assay .

方法；通过 色 氨 酸酶活力测定，考察三种固定化材料及温度、pH、单价阳离子和乙醇对固定化 WW－11 色 氨酸酶活力的影响。

Synthesis of SOPC and Its Application to Activity Assay of Tryptophanase

SOPC的合成及其在 色 氨 酸 酶活力测定中的应用

Molecular Cloning and Expression of Tryptophanase Gene of Escherichia coli

大肠杆菌 色 氨 酸 酶基因的克隆与表达

Study on Immobilization of Tryptophanase Genetic Engineering Strain and Its Culture Conditions

色 氨 酸 酶基因工程菌固定化及其培养条件的研究

The optimum pH and temperature of degradative reaction by tryptophanase of immobilized WW-11 were 9 . 0 and 50 ℃ .

固定化WW－11 色 氨 酸 酶降解反应最适pH为9.0、最适温度为50℃；

Conclusion : The stability of tryptophanase of immobilized engineering strain by temperature and alcohol was superior to that of free strain .

结论：固定化工程菌 色 氨 酸 酶对温度、乙醇等的稳定性比游离菌有显著 提高。

Results : PVA served as immobilized carrier of NO. WW-11 and its activity recovery of tryptophanase was 60 . 9 % .

结果：以聚乙烯醇作为 WW－11的固定化载体，其活力回收为60.9%。