Methods 18 mer nucleic acid was synthesized and complementary to the translation initiation site of mRNA of dipterex resistant mosquitoes . The ODNs were annealed to the corresponding mRNA molecules and they were added to rabbit reticulocyte cell free system . The translation products were analyzed by SDS PAGE .

方法 人工 合成互补于抗性库蚊酯酶mRNA 翻译起始点18碱基，与淡色库蚊mRNA退火后加入无细胞翻译体系，进行体外翻译，翻译产物用SDS聚丙烯酰胺凝胶电泳进行分析。

Methods Three 20 mers phosphorothioate oligodeoxynucleotides ( ODNs ) were synthesized : ( 1 ) Cyclin D1 antisense ODN ( AS ODN ) targeting the initiation site of translation in cyclin D1 mRNA ;

方法 合成针对细胞周期素D1mRNA 翻译起始部位序列的20bp硫代磷酸反向寡脱氧核苷酸（AsODN）。