ultracentrifugation

[ʌltrəsentrɪfjʊ'ɡeɪʃn][ʌltrəsentrɪfjʊ'ɡeɪʃn]

超速离心法

  • Conclusion : We can prepare the coating antigen in measles IgG EIA test system by continuous sucrose density gradient ultracentrifugation .

    结论:采用超滤、 PEG 沉淀 连续蔗糖密度梯度 超速 离心的方法可制备满足麻疹IgGEIA 诊断试剂要求的包被抗原。

  • The liposomes and free drug were separated by Sephadex filtration dialysis and refrigerated ultracentrifugation respectively then the entrapment efficiency of the liposomes was calculated .

    分别采用葡聚糖凝胶过滤法、透析法、 超速冷冻 离心 分离脂质体和游离药物,计算脂质体包封率。

  • In order to study the physical-chemical properties and functions of LDL researchers have invented a variety of LDL separation methods such as ultracentrifugation chemical precipitation gel filtration and affinity chromatography . However each of them has its disadvantages .

    为研究LDL的理化性质及其功能,研究人员发明了多种LDL分离技术,如 超速 离心、化学沉淀、凝胶过滤层析及亲和层析等,但这些技术均有不足。

  • An Analytical Ultracentrifugation Method for the Measurement of Molecular Weight and Sedimentation Coefficient of Biological Macromolecules

    分析 超速 离心法测定生物大分子的沉降系数和分子量

  • The molecular weight was determined to be 280 000 by ultracentrifugation .

    离心 分析 相对分子质量为 280000

  • The ser - um could be separated into 8 layers by potassium bromide density gradient ultracentrifugation .

    经溴化钾密度梯度 离心分离大 血清得到8层 制品

  • Objective To prepare a kit for detecting apoprotein ( Apo ) A1 and B. Methods Dextran sulfate precipitation ultracentrifugation chromatography and so on .

    目的研制载脂蛋白A1和B检测试剂。方法采用硫酸葡聚糖沉淀、 超速 离心、层析等方法。

  • The various lipoproteins in rabbit serum may be separated by ultracentrifugation in a vertical rotor at once .

    用垂直 转头 超速 离心 一次可 备兔血清各种脂蛋白。

  • Rapid Isolation of Large Amount of Plasma VLDL and LDL by a Two Step ultracentrifugation

    两步 超速 离心 快速分离大量血浆极低密度脂蛋白及低密度脂蛋白

  • METHODS : TAF was purified at scheduled intervals by using extract methods such as hollow fiber column ultracentrifugation chromatography and electrophoresis .

    方法:应用中空纤维超滤、 超速 离心、层析和电泳等方法,对肺癌及卵巢癌患者的胸腔积液、腹水中TAF进行提纯和分析;

  • Methods ( 1 ) Plasma lipoproteins were isolated and purified by ultracentrifugation .

    方法(1)用 离心 方法分离纯化血浆各脂蛋白;

  • Results of ultraviolet spectrum analysis sucrose gradient ultracentrifugation and protein synthesis activity in wheat-germ cell-free system showed that the polysomes maintained their integrity with rather high protein synthesis activity .

    紫外光谱和蔗糖梯度 离心分析及麦胚无细胞体系翻译活力测定表明制备过程中Rp基本保持完整,有较高的促进放射性氨基酸参入活力。

  • Purifying the RHDV which has already been purified firstly by methods of ion exchange chromatography gel chromatography density gradient ultracentrifugation and examining the content of RHDV by analyzing HA and protein degree examining the pureness by the test of ELISA and SDS-PAGE .

    运用离子交换层析法、凝胶过滤层析法和密度梯度 离心 对已作初步处理的RHDV分别进行提纯,并通过血凝性鉴定、抗原蛋白含量检测、ELISA和SDS-PAGE凝胶电泳等方法检测其纯度。

  • Monomeric and trimeric subcomplexes of LHC ⅱ were obtained by using sucrose gradient ultracentrifugation .

    经蔗糖密度梯度 超速 离心获得了该色素蛋白复合体的单体和三聚体。

  • Methods : To isolate LDL by using polyanions precipitation and short-time density gradient ultracentrifugation .

    方法:采用多聚阴离子沉淀结合短时密度梯度 超速 离心 得到纯化的LDL;

  • Rapid fractionation of polysomes by density gradient ultracentrifugation in vertical tube rotor

    用垂直转头密度梯度 超速 离心 快速分级分离多聚核糖体

  • Determination of Protein Sedimentation Coefficient by Sucrose Density Gradient Ultracentrifugation

    蔗糖密度梯度 离心 测定蛋白质的沉降系数

  • The 3 % LM-solubilized fractions are separated by different methods : sucrose density gradient ultracentrifugation anion exchange column molecular size elution column .

    对于3%LM增溶后的类囊体膜蛋白,分别采用了蔗糖密度梯度 离心,离子交换柱层析和分子筛层析等分离纯化方法。

  • This protein could be purified with CsCl and sucrose density gradient ultracentrifugation .

    CsCl 超速 离心结合蔗糖密度超速 离心可纯化此蛋白。

  • BALB / C mice were immunized with infectious bursal disease virus ( IBDV ) which had been purified with ammonium sulfate precipitation high-speed centrifugation and ultracentrifugation in a sucrose gradient .

    用经硫酸铵沉淀、高速离心并 蔗糖 密度梯度 离心提纯的鸡传染性法氏囊病病毒(IBDV)免疫BALB/C小鼠。

  • Isolation of human serum lipoprotein $ by density gradient ultracentrifugation in a zonal rotor & ⅰ . isolation of large amounts of human serum very low density and low density lipoproteins

    人血清脂蛋白的 超速 离心分离&Ⅰ.人血清极低密度及低密度脂蛋白的大量分离

  • Conclusion Modified ultracentrifugation is more suitable for preparing the sample of central nervous system .

    结论改良 超速 离心 更适于制备 来源于中枢神经系统 双向 电泳样品。

  • A study of forming niobium colloid in nitric acid solution by ultracentrifugation

    离心 研究硝酸溶液中铌胶体的形成

  • The results show that the density difference between particles and gradient medium particle size and surface chemistry plays a major role in the density gradient ultracentrifugation rate separation .

    研究表明在 离心过程中,颗粒与梯度介质密度差、颗粒尺寸及其表面化学对其在密度梯度里分离起主要作用。

  • Methods Exosomes were purified by ultrafiltration centrifugation and sucrose gradient ultracentrifugation . The expression of HSP70 、 ICAM-1 、 ABL were investigated by solid phase immunoelectron microscopic method ( SPIEM ) .

    方法离心超滤和蔗糖密度梯度 离心 分离K562细胞释放的exosomes,固相免疫电镜法(SPIEM)制备exosomes的HSP70、ICAM-1及ABL免疫电镜标本。

  • In the first part of our experiments exosome derived from 5 CML patients and K562 cells were purified by ultrafiltration centrifugation and sucrose gradient ultracentrifugation .

    第一部分实验,采用离心超滤和蔗糖密度梯度 离心方法分离出5例慢性髓细胞白血病慢性期患者CML细胞和K562细胞释放的exosome,经透射电镜观察其超微结构。

  • The disease-specific protein of rice grassy stunt virus ( RGSV ) was purified by differential pH precipitation and ultracentrifugation .

    采用不同pH值沉淀法及 超速 离心获得提纯的水稻草矮病特异蛋白。

  • Methods : SD rats male were used to prepare the rat liver microsomes by using differential ultracentrifugation .

    方法:SD大鼠,雄性, 断头处死 肝脏制成匀浆,差速 离心法制备肝微粒体。

  • This method is simple needs only small amount of serum and no ultracentrifugation .

    本法操作简便、血清用量小、不需 超速 离心

  • Apolipoprotein C ⅰ C ⅲ were isolated and purified by ultracentrifugation gel filtration and high performance liquide chromatography from human serum very low density lipoprotein .

    本文 应用 超速 离心、凝胶过滤层析、高效液相色谱法分离纯化了人血清极低密度脂蛋白&载脂蛋白CⅠ和CⅢ。