Influence on drug yields in rabbit liver and blood after trypsin digestion combined with EDTA

胰 蛋白酶 消化 法合用乙二胺四醋酸二钠对兔肝和血中药物得率的影响

Peptide map was determined by trypsin digestion and followed RP-HPLC analysis .

胰酶 消化及RP&HPLC法测定肽图；

Eight sheep fetal fibroblast lines and four female adult Dorset ear fibroblast lines were established by trypsin digestion and tissue attached culture method respectively .

采用 胰酶 消化 法和组织贴壁法分别建立了乙个绵羊胎儿成纤维细胞系sFFI－－8和4个雌性成年Dorset绵羊耳部成纤维细胞系DFI－4。

To culture human conjunctiva fibroblast cells by trypsin digestion method and describe their growth features in vitro .

方法：1、用 胰 蛋白酶 消化 法取材人结膜成纤维细胞，并描述人结膜成纤维细胞的生物学特性。

The embryos were manually taken from the Nile Tilapia at somite differentiation stage . The ECs derived from the Nile Tilapia were isolated and sub-cultured with the trypsin digestion .

从发育至体节分化期尼罗罗非鱼手工分离出胚胎，采用 胰 蛋白酶 消化 法对尼罗罗非鱼ECs进行原代和继代分离培养。

The ECs derived from the zebrafish at gastrula stage were isolated and sub-cultured with the trypsin digestion .

采用 胰 蛋白酶 消化 法从原肠期斑马鱼分离出ECs，并进行原代和继代培养。

The relationship between the trypsin digestion of bone collagen and the component content of gelatin

胰 蛋白酶 降解骨胶原与明胶组份含量之间的关系

Methods Human DAF was purified from human erythrocyte ghost by trypsin digestion butanol extraction sequential chromatography on DE32 and immunoaffinity chromatography .

方法经 胰 蛋白酶 消化、正丁醇抽提及DE32离子交换色谱和抗体亲和层析等步骤从人红细胞膜影中分离纯化人红细胞膜DAF。

Two kinds of pure cells were obtained by trypsin digestion and culture with serum containing and serum free medium .

二种方法均用 胰酶不 完全 消化和加入血清或无血清培养相结合的方法得到纯化的内皮细胞。

The differential protein spots in the gels were cut then the peptide mass fingerprint were obtained through In-gel trypsin digestion and matrix-assisted laser desorption / ionization time of flight mass spectrometry ( MALDI-TOF - MS ) analysis . 5 .

将凝胶中的差异蛋白点切出，经胶内 胰 蛋白酶 解和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）分析获得肽质量指纹谱。

CFs were isolated by trypsin digestion method from Wistar rats and identified by invert microscope and vimentin immunofluorescence . 2 .

方法：1.采用 胰酶 消化法获得Wistar大鼠CFs。采用倒置相差显微镜和波形蛋白免疫荧光法鉴定细胞。

METHODS CFs were isolated by trypsin digestion method .

方法 胰酶 消化 法分离、培养新生SD大鼠CFs，采用 硝酸 还原酶 法和分 光 光度法分别测定基础状态和AVP 干预下CFs的 NOS 活性和NO 含量。

Low concentration and shortened time of trypsin digestion and mechanical dissociation were adopted to conduct culture .

方法：采用低浓度、短时间 胰酶 消化和机械分离相结合的方法 制 备 皮层 神经元 悬 液，进行培养。

After culture 20-25 days combined with a mild trypsin digestion method and orbit shaking method to purify astrocytes .

培养20-25天，用温和 胰酶 消化结合定轨摇床振荡的方法分离纯化星形胶质细胞。

Methods We isolated and cultured cardiomyocytes in neonatal mice by trypsin digestion technique . The cells were inoculated in 96-well culture plate at the density of 1 × 10 5 / ml.

方法 胰酶 消化法分离和培养KM乳鼠心肌细胞，按照1×105ml的密度接种于96孔培养板。

METHODS The peptide mapping was analyzed by trypsin digestion and RP-HPLC analysis .

方法采用 胰 蛋白酶 裂解 -反相高效液相色谱法测定肽图；

The corresponding antigen was sensitive to trypsin digestion .

此抗体对 E 玫瑰花 生成有明显 阻断 作用，其相应抗原又与 E 受体 一样对 胰酶 消化敏感。

The antibody activity reduced by 50 % retention rate 8 hours after trypsin digestion After exploring the appropriate amount and the best therapeutic approach were optimised .

胰酶 消化8小时后，抗体活性保留率为50%。经过试验，找到了抗体 液的最适用量和最佳治疗途径。

Then using the improved explant and trypsin digestion methods buffalo fetal fibroblast cells were efficiently isolated from the fetal 's skin and had been cultured in vitro for three generation .

然后，用经过改良的组织块法和 胰酶 消化法从水牛胎儿肌肉组织中成功分离得到水牛胎儿成纤维细胞，体外培养至第三代。

Methods CFs were isolated from neonatal SD rats by trypsin digestion and growth-arrested CFs were stimulated with Sim and / or MVA at varied concentrations for different time lengths and the DNA synthesis in the cells was measured by 3H-thymidine ( 3H-TdR ) incorporation assay .

方法采用 胰酶 消化、差速贴壁法培养新生SD大鼠CFs，以3H-胸腺嘧啶核苷（3H-TdR）掺入法测定DNA合成，观察不同浓度Sim及MVA分别作用不同时间对CFsDNA合成功能的影响。

Methods of trypsin digestion to separate MEF of ICR mouse which can be purified by subculture without using other methods and can used to prepare feeder layer . 2 .

结论1. 胰 蛋白酶 消化法分离ICR小鼠胚胎成纤维细胞，经传代培养即可纯化，无需其他的方法纯化MEF，可用于制备饲养层。

T_1 is degraded gradually by trypsin digestion and simultaneously loses its capacity to combine with the corresponding receptor .

胸腺激素与受体的结合能力与它的生物活力是相关的，T1的活力随 胰 蛋白酶 的 消化而逐渐丧失，相应的T1与受体结合的能力亦逐渐降低至零。

We have modified the procedure to a sequential digestion of papain and DNase I for taking into account the fast traditional trypsin digestion .

考虑到传统的 胰酶 消化速度不好控制，我们用木瓜酶结合DNA酶I序贯消化。

Dispase isolated enzymes and Trypsin digestion two-step method was used to prepare and cultivate normal human foreskin keratinocytes . 2 .

方法：1.采用Dispase分离酶、 胰 蛋白酶两次 消化法制备和培养正常人包皮组织角质形成细胞。

Mechanism and Kinetic Model for Limited Trypsin Digestion of Protein in Rice Dregs

胰 蛋白酶有限 酶解米渣蛋白的机理及动力学模型研究

Results : 1 . Bone marrow culture-wide ( that is law adherent ) Isolation and Purification of goat MSCs by trypsin digestion for liquid and gradually purified after passage method is simple and practical . 2 .

结果：1.采用全骨髓培养法（即贴壁法）分离纯化骨髓间充质干细胞，经换液和 胰酶 消化传代后逐渐纯化，方法简单实用。

The medium was changed every two days time until the cell grew to80 % by trypsin digestion and passage .

每2天换液一次，细胞生长至80%时用 胰 蛋白酶进行 消化传代。

METHODS Osteoblastic cells were isolated from newborn rat calvaria through trypsin digestion .

方法原代培养新生大鼠 颅盖骨成骨细胞，3H胸腺嘧啶掺入法测定成骨细胞的增殖；