Advances in exploring localization and biopsy of sentinel lymph node with vital dye

染色法定位 前哨淋巴结活检的研究进展

AIM : To explore the application value of vital dye CFDA SE to study of lymphocytic proliferation .

目的：探讨 活体 染料CFDASE在淋巴细胞增殖研究中的应用价值。

Application of vital dye CFDA-SE in transplant immune research

羧基 荧光素 乙酰乙酸在移植免疫研究中的应用

The modified method of collagenase enzyme digestion-density gradient centrifugation procedure yielded a preparation primarily consisting of cells nearly the same volume with ~ 95 % viability as assessed by exclusion of the vital dye trypan blue .

采用改良的 胶原酶消化-密度梯度离心法获得的细胞大小一致，活性>95%；

Application of vital dye CFDA-SE to study lymphocytic proliferation

活体 染料CFDA-SE在淋巴细胞增殖研究中的应用

CONCLUSION : Our results show that application of vital dye CFDA-SE and SNARF-1 combined with flow cytometry is a powerful tool for evaluation of responsive intensity in one-way MLR .

结论联合应用CFSE和 SNARF-1 染色，结合流式细胞术，可作为评价混合淋巴细胞反应中应答细胞的应答强度的有力工具。

Macrophages phagocytize foreign materials such as dust and carbon particles in the lung as well as vital dye particles .

巨噬细胞吞噬异物，如肺内的尘埃及碳粒，同样还有 活体 染料的微粒。