To reduce false positive rate of peptide identification from tandem mass spectra thresholds of search engine filters were raised which in turn leads to high false negative rate and greatly reduces proteomics research efficiency .

在应用 二 级 质谱图鉴定多肽时，为了降低鉴定的假阳性率，通常会提高 检索软件的阈值设定，这种行为导致了多肽鉴定的假阴性率的提高，大大降低了蛋白质组研究的效率。