Because the fusion protein expressed in E. coli has6 × histidine tag it was purified with Ni-NTA affinity column after lysis of inclusion bodies with urea .

由于在大肠杆菌细胞中表达的融合蛋白带有六组氨酸 标签，所以在使用尿素对包涵体进行溶解 后，通过Ni-NTA亲和层析柱纯化了AT4G12490重组蛋白。

However these cognitive linguistic theory can only partly explain the allegorical meaning of construct but no one is concerned about the tag line break after the internal reasons .

然而，这些认知语言学理论只能部分地解释歇后语的意义构建，很少有人关注 歇后语歇 后的内部原因。

Results : A DNA fragment encoding conservative domain of E1 amplified by PCR was inserted into the downstream of 6 × His tag of pEK-318 vector . His-E1 ( 14kD ) was purified by affinity chromatography after induced expression in E.

结果：将PCR扩增的E1蛋白保守功能域（第362～456aa）基因片断重组于原核表达载体pEK-318的6×His 标签下游， 经诱导表达和亲合层析，纯化出14kDHis-E1蛋白。

Without such contingents the best a nation can do is to tag along after other nations but in that way you cannot score major breakthroughs in science and technology .

如果没有这样一支队伍，在科学技术上至多只能步别国的 后尘，很难取得重大的突破。

RFID tag data acquisition is simulated for marshalling yard using the program developed with Microsoft Visual Studio after the marshalling yard data acquisition framework based on RFID is constructed .

在方案设计的基础上，构建了基于RFID的编组站管控系统数据采集系统架构，并利用MicroSoftVisualStudio开发的RFID 标签数据获取程序对编组站 标签数据获取进行了模拟。

Anthropologist Edward Smith recalls that when he worked as a White House speechwriter there was a rule against wearing the White House tag after work .

人类学家 爱德华·史密斯记得，当他在白宫担任讲稿撰写人的时候，白宫曾有规定禁止其雇员在 下班 以后继续佩戴胸 牌。

When we detect an open bracket we have found the start of a tag and return the collected characters after we have reversed their sequence .

当检测到一个开括号时，我们就找到了一个 标签的开头，并将收集到的字符 在逆转顺序 之后返回。

You activate the code by adding an onload method to the tag which starts the code right after the page finishes loading .

可以通过向 标记添加onload方法来激活代码，此方法会在页面加载 之后立即启动代码。

The audio converter supports ID3 tag all ID3 tags in the original files will be reserved after the audio converting processes .

在音频转换器支持的ID3 标签，所有的ID3标签中的原始文件将保留 在音频转换过程。

As you can see we have separated each tag proposal into two parts : in a part before the planned cursor position and in a part after the planned cursor position .

可以看到，我们将每个 标签建议划分为两个部分：一部分在预计的光标位置之前，一部分 在预计的光标位置 之后。

It might be tempting to set some sort of timer before creating the dynamic script tag and just assume the JSON has arrived after say three seconds .

在创建动态 标记之前设置某种定时器，然后假定JSON在某个时间，比如说3秒 之后到达，这种方法初看起来似乎可行。

2 ) The first ATG rule - ORF initiates from the first in-frame ATG codon in the DNA sequence after the nearest upstream terminator ( TAA TAG or TGA ) .

2）第一ATG规则&ORF起始于上游的终止密码子（TAA， TAG或TGA） 后第个同框架的ATG。

Last week I 've forgotten to wear my watch to work I left behind my staff tag after work and I also took the wrong turn on my way to get breakfast .

上个星期我有忘记戴手表出门，忘了把员工 证 带 回家，忘了买早餐的路该怎么走。

Application developer can use this Tag time after time to application systems thereby to implement information system with workflow character rapidly which can markedly shorten the cycle of the development critical business applications .

应用开发者可以重复利用此 标签 在自己的应用系统中从而快速实现工作流性质系统，这样可以显著地缩短关键业务的开发周期。

For the purpose of the correct use of this kind of specific question this paper make somewhat of a thorough collection of various tag questions analyzing them one after another so that a satisfactory explanation would be presented .

为了正确地使用这种特殊的问句形式，本文收集了各类常用 附加疑问句，通过 逐一分析，以期对这一语言现象作一较为全面的解释。

Drag and drop the static page container onto the static layout file inside of the content area tag just after the tag

将静态页面容器拖放到内容区域 标签的镜头布局文件中，该标签位于 标签 之后

In passive UHF RFID tag chip an embedded NVM is required to store the information such as security information electronic product code or user information after power off .

而非易 失性存储器又是射频识别 标签的信息载体，主要用于安全信息、产品编码、用户信息等数据的存储。