In this part a novel fluorescent method of protein detection has was developed using hairpin aptamer based on polymerase reaction . The hairpin aptamer was designed to be employed as the protein ligand and template of the polymerase reaction .

本部分设计了一种新型的发夹型核酸适体探针，利用其与靶蛋白反应后构象 发生变化的特点，建立了一种基于 聚合酶 反应的荧光信号放大的蛋白质检测新方法。

Applied FeSO_4 · 7H_2O and ( NH_4 ) _3PO_4 · 3H_2O as materials PEG-400 as template multi-micronutrient fertilizer ferrous ammonium phosphate was synthesized by low heat solid state reaction .

以FeSO4.7H2O和（NH4）3PO4.3H2O为原料，用聚乙二醇-400作 模板剂，经低热固相 反应合成了磷酸亚铁铵多元微肥。

The results showed that the insert fragment length of target clones as λ phage DNA template directly sequencing was longer than that of PCR products after recycling but a lower succeeding ratio for sequencing reaction .

结果表明，以λ噬菌体DNA为 模板直接测序比PCR产物经回收后为 模板进行测序，其目标克隆的平均插入片段长度要长，但 反应成功率低。

The major results are as follows : 1 . Compare the different effects of the dosage of template DNA Mg2 + dNTPs and Taq enzyme in PCR amplification then establish an ideal PCR reaction system to test cucumber hybrids purity . 2 .

主要研究结果如下：1.比较 模板DNA、Mg2+、dNTPs和Taq酶的不同用量对PCR扩增的影响，建立了适用于黄瓜杂种纯度检测的理想PCR 反应体系。

A rapid simple and reliable method to prepare dsRNA template for reverse transcription polymerase chain reaction is established .

应用 RT－PCR技术检测草鱼出血病病毒。建立了一种快速、简易而可靠的dsRNA 模板的制备方法。

The optimum annealing temperatures of 51 ° C - 55 ° C were definite after selecting and optimization of the primer combinations for SRAP with the DNA pools from male and female plants of Ginkgo as a template in the stable reaction system obtained . 5 .

以银杏的雌雄株DNA池为 模板，用获得的稳定的 反应体系对sRAP引物组合进行筛选优化，得到不同引物组合的最适退火温度，退火温度的范围在5l℃-55℃之间。

Then the positive TTV DNA was used as a template to optimize nested PCR reaction conditions . The methods were verified through repetition sensitivity and specificity of tests .

再用阳性TTVDNA为 模板，对巢式PCR 反应条件进行优化，通过重复性、敏感性、特异性试验来验证该方法的准确性。

Results For LCN template DNA if only increased the amount of Taq enzyme or the reaction volume the results of STR amplification were scarcely improved but if increased PCR cycles the detection sensitivity were significantly improved .

结果对低拷贝 模板DNA，单纯增加Taq酶量或 反应 体系，扩增效率改善不明显；增加循环数，显著提高检验灵敏度；

CD was cultured successfully and the genome was extracted as template of PCR reaction . 2 .

获得如下结果；1.成功的培养了艰难梭菌，并提取了其基因组作为PCR 反应 模板。

A novel method for synthesis of sodium aluminium carbonate hydroxide was studied and the target product was obtained with aluminium sulfate as aluminium source and PEG-400 as template via the one step low heat solid state reaction .

对合成碱式碳酸钠铝的新方法进行了研究，以聚乙二醇400（PEG400）为 模板，硫酸铝为铝源，用低热固相 反应一步法成功合成得到碱式碳酸钠铝。

Using the DNA extracted from bone marrow smears which have been stored routinely as a template polymerase chain reaction ( PCR ) was utilized to detect clonal immunoglobulin heavy chain ( IgH ) gene rearrangements in multiple myeloma ( MM ) and reactive plasmacytosis ( RP ) .

从常规保存的骨髓涂片抽提DNA为 模板，应用聚合酶链 反应（PCR）技术检测多发性骨髓瘤（MM）与反应性浆细胞增多症（RP）IgH基因重排方式。

The effect of different concentrations of template DNA primer dNTPs and Mg2 + on the PCR reaction was analyzed and the optimized RAPD and ISSR-PCR systems in radish were established .

对影响PCR 反应的主要因子& 模板DNA、引物、dNTPs和Mg2+浓度进行优化，建立起适合于萝卜基因组DNA的RAPD和ISSR反应体系。

Preparation of Single-Stranded DNA Template for Pyrosequencing by Linear-after-the-Exponential-Polymerase Chain Reaction

指数线性聚合酶链式 反应法制备焦磷酸测序 反应的单链 模板

Methanol method for the preparation of template DNA from blood for polymerase chain reaction

甲醇法制备的血 模板DNA用于聚合酶链 反应

The max magnetic saturation magnetization of magnetic hybrid microsphere is 5.2emu/g when the ratio of iron salt to template spheres is 0.162 in the co-precipitation reaction .

当共沉淀 反应中铁元素与 模板微球的质量比0.162时，磁性微球具有最高的饱和磁化强度，为 5.2emu/g。

Then the template substrate reaction with different boric acid boric acid and ester reaction of pyridine the corresponding yield obtained to study the law .

以此优化的条件为前提，再将该 模板 反应的底物与不同的硼酸，吡啶硼酸和酯反应，得出相应的产率，研究其规律。

Using potassium titanium oxalate as raw material ring-like TiO2-aggregates film deposited on the glass substrate was successfully synthesized by hydrothermal method though a bubble template strategy and ligand exchanging reaction .

采用水热法，以草酸钛钾为反应原料，借助气泡 模板和配体交换 反应，在玻璃基底上成功沉积了环状的Ti02聚集体薄膜。

Recombinant PUC inserted with full length nucleic acid sequence of chrysanthemum stunt viroid was used as template DNA to prepare digoxigenin-labelled and biotin-labelled probes with two universal primers by polymerase chain reaction .

以含菊花矮化类病毒（Chrysanthemumstuntviroid.CSV）全长序列的重组质粒PUC为 模板DNA，加入通用引物，在聚合酶链式 反应系统中制备地高辛标记的CSVcDNA探针和生物素标记的CSVcDNA探针。

The results showed that : the template method combined with ultrasonic in two steps . The reaction was at 60 ℃ under ultrasonic for 3h and molar ratio of catechol to 1 3 - dibromo propane was 1:1 .

结果表明：用超声波法与 模板法结合分两步加料，在60℃的 反应条件下超声波 反应3h，采用邻苯二酚同1二溴丙烷总的摩尔比为1：1分两步加料。

A simple and rapid method was used to prepare template DNA for polymerase chain reaction Cells and minced tissues were digested with proteinase K.

在多聚酶链 反应时采用一种快速简便的 模板DNA制备方法。组织块在冰浴中剪碎和匀浆，再用蛋白酶K降解；

This model describes the function relation between product quantity or fluorescence intensity and initial template number and other reaction conditions and can reflect the accumulating rule of PCR product molecule accurately .

PCR动力学数学模型是根据PCR技术的原理提出的，能够准确描述PCR反应产物分子数量积累规律的动力学方程，给出了PCR产物数量或者荧光强度与初始 模板数量及其他 反应条件间的函数关系。

Methods w_3698 As a template the human genome DNA was extracted from healthy human blood firstly . And then the polymerase chain reaction ( PCR ) was applied to clone the 2 335 bp fragment of human midkine promoter gene .

方法自健康人血中提取人类基因组DNA作为 模板，聚合酶链式 反应（PCR）技术克隆人MK启动子基因2335bp片段。

Through coordinating organic ligands metal particles template and controlling reaction conditions organic skeleton materials of porous metal will be likely produced .

通过调节有机配体、金属离子、 模板 剂和控制 反应条件可能定向组装出预期的多孔金属有机骨架材料。

Acetonitrile as solvent when the molar ratio of template functional monomer and cross linker is1:8:8and the reaction temperature is60 ℃ the imprinted polymers possess optimal selectivity and adsorbability .

以乙腈为溶剂， 模板、功能单体和交联剂的物质的量比为1：8：8时， 反应温度为60℃时制备的印迹聚合物具有最佳的选择性、吸附性。

And the catalysts for the reaction were optimized through comparison of experimental template reaction .

并通过对 模板 反应的对比实验，对反应中加入的催化剂进行了优化。

S A method of rapid detecting Leptospira DNA was constructed after the template DNA from 4 serotypes of Leptospira interrogans were successfully amplified by polymerase chain reaction ( PCR ) .

以四株不同型别的致病性钩端螺旋体DNA为 模板进行聚合酶链 反应（PCR），建立了钩体快速检测方法。

The anomalous typing could result from a lot of causes including DNA genetic variation poor quality or quantity of DNA template different typing system or method nonspecific reaction in PCR or anomalous electrophoresis migration .

引起这些异常现象原因很多，有DNA的遗传性变异、DNA 模板质量不好、分型试剂或方法的不同、PCR反应中非特异性 反应以及异常的电泳行为等。