In recent years MHC-peptide tetramer technology has been developed to directly ex vivo visualize antigen-specific T cells efficiently and becomes the critical approach in studying T-cell immune responses .

MHC-肽 四 聚 体技术是直接检测抗原特异性T细胞的有效而特异的方法，成为目前研究T细胞免疫应答的重要技术之一。

AIM : To study the biological activity of tetramer of bispecific single-chain antibody against human prostate-specific antigen ( PSA ) and CD3 molecule .

目的：研究抗前列腺特异抗原（PSA）/抗CD3 双特异性单链抗体 四 聚 体的生物活性。

Preparing of the single-chain MHC-peptide complex tetramer

可溶性单链 MHC-肽 四 聚 体的构建研究

Detection of HBV specific cytotoxic lymphocytes responses in severe hepatitis B patients using MHC-peptide tetramer flow cytometry

重型肝炎患者乙型肝炎病毒特异性细胞毒性T淋巴细胞 应答功能研究

We suggest that the octamer of molybdenum-iron protein is a possible metablite in regulation of nitrogenase activity the conversion from the tetramer to octamer will cause the decrease of nitrogenase specific activity in vivo .

我们推断，生长过程中胞内 钼铁蛋白聚合态的变化可能是调节固氮酶活力的一种方式。

260 protein was purified to homogeneity in a two-step procedure of Ni2 + chelating and size exclusion chromatography . The purified protein exists in two forms a dimer form about 46 ku with yellow color and a tetramer form without apparent color .

260蛋白通过Ni2+亲和柱和分子 筛两步法纯化，并发现纯化后的蛋白以两种形式存在，二聚体（约46ku）和 四 聚 体， 前者呈 亮黄色， 后者无色。

Biotinylated HLA-A0201 / peptide complex monomer was labeled with streptavidin ( including four biotin sites ) fluorescein ( phycoerythrin or allophycocyanine ) by the ratio of 4:1 to form soluble HLA - A 0201 / PR1 tetramer .

生物素化的HLA-A0201/肽复合物单体与荧光素（phycoerythrin或allophycocyanine）标记的链霉亲和素（含4个生物素化位点）按4∶1的比例结合，形成可溶性 HLA-A0201/PR1 四 聚 体。

Conclusion HLA-peptides tetramer staining flow cytometry is a direct exvivo method to detect the lever of circulating HBV specific CD8 + cells which reflects the response of CTL and relates to clinical consequence in hepatitis B patients .

结论HLA 肽 四 聚 体流式细胞技术能在体外直接检测HBV特异性的CD8+T细胞数量，其水平一定程度上反映了特异性CTL应答状况，且与乙肝的不同临床转归有关。

The experimental results showed that Rhus Laccase polymerized the guaiacol to various low-molecular-weight polymers two of which were identified to be tetramer and pentamer .

结果表明：漆树酶能使愈创木酚聚合为低分子聚合物，其中2种产物经鉴定为 愈创木酚的 四 聚 体和五聚体。

The molecular weight of BSL is 56 kd . It is a tetramer 1 β 3 which consists of 2 subunits with one 16 kd (α) and three 13.6 kd (β) polypepeides .

BSL的分子量为56kd，由分子量分别为 13.6kd（β）和16.0kd（α）的两个不同的亚基以α1β3所组成。

Construction of a BF2 / Peptide Tetramer and Its Application on the Detection of Specific T Cell

BF2/肽 四 聚 体的构建及其在特异性T细胞检测上的应用

As a dehydrogenase GDH exists wildly in the animals plants and microorganism . The enzyme exists in the form of tetramer or hexamer and plays a very important role in the metabolism of carbon and ammonia .

作为一种脱氢酶，它广泛的存在于各种动植物以及微生物体内，常以 四 聚 体或六聚体的形式存在，在生物体的碳、氮代谢中起着非常重要的作用。

MHC peptide tetramer is a sensitive method to evaluate antigen specific CTL .

MHC-肽 四 聚 体 技术 是 近年来建立的检测特异性CTL的有效方法。

Quantitation of antigen specific T lymphocytes in peripheral blood of patients with primary biliary cirrhosis using tetramer technology

四 聚 体技术检测原发性胆汁性肝硬化患者血循环中抗原特异性T细胞

Application of MHC-peptide Tetramer Technology in Epitope Identification

MHC-肽 四 聚 体技术在CTL表位鉴定中的应用

The octamer formation in vivo is related to cell growth process . During the log-phase of cell growth the specific activity of nitrogenase is increasing and the tetramer is the major form of molybdenum-iron protein .

在细胞生长过程中，胞内钼铁蛋白两种聚合体的相对含量出现规律性变化：在对数期，细胞固氮酶比活力 成上升趋势，而 钼铁蛋白主要以高活力的 四 聚 体形式存在；

CONCLUSION : The optimized tetramer staining condition can increase the specificity of tetramer staining and decrease unspecific binding therefore it is applicable for phenotyping and functional analysis of antigen-specific CTL .

结论： 通过对染色条件进行优化可明显提高四聚体染色的特异性，降低非特异性结合，优化的 四聚 体染色法能用于抗原特异性CTL的表型和功能分析。

Biological activity of tetramer of bispecific single-chain antibody against human prostate-specific antigen and CD3 molecule

抗前列腺特异抗原/抗CD3 双特异性单链抗体 四 聚 体的活性研究

Construction of recombinant vector MHC - ⅰ - fluorescin tetramer and its eukaryotic expression

MHC-Ⅰ荧光 四 聚 体载体的构建及其真核表达

E. the acid volume of the catalyst increased the conversion for isobutene and the selectivity for trimer increased also while the selectivity for dimer decreased and the selectivity for tetramer was almost unchanged .

增加时，即催化剂的酸量增加时，异丁烯的转化率和三聚物的选择性也增加，但二聚物的选择性降低， 四 聚 物的选择性则几乎没有变化。

Preparation and identification of HBc-HLA-A ~ 1101 - β _2m complex monomer and tetramer

HBc-HLA-A~1101-β2m复合物单体及其 四 聚 体的制备和鉴定

The purpose of this review is to describe the rationale and method of tetramer technology then to focus on the use of tetramer technology to study virus infection anti-tumor actions and autoimmune disease .

本文就 MHC- 肽 四 聚 体技术的基本原理、 制 备方法，以及在病毒感染、肿瘤、自身免疫性疾病等研究领域的应用进展作一综述。

The Effects of Substrates and Inhibitor on the Submolecular Structure of Acetylcholinesterase Tetramer Incorporated in a Mica-supported Artificial Phospholipid Membrane

底物及抑制剂对重组在人工磷脂膜上的 乙酰胆碱酯酶 四 聚 体亚分子结构的影响

The high benzyl methyl silicone oil could be obtained through polymerization of two benzyl mono - mers and D_4 cyclic tetramer .

用2种苯基单体和D4环 体聚合，就可得到高苯基甲基硅油。

In order to increase the stability of hemoglobin tetramer relationship of hemoglobin 's structure and function were studied with computer modeling approach .

为了研究提高血红蛋白 四 聚 体的稳定性，利用计算机 同源模 建、 分子间 氢键形成及表观 静电势理论，探讨血红蛋白及相应突变体的结构与功能关系。

Previously used methods for detecting specific CTL are usually indirect . In recent years tetramer technology has been developed to directly visualize antigen-specific CTL efficiently and become the critical approach in studying T cell immune responses .

而过去检测CTL的方法都是间接的，最近发展起来的 四 聚 体技术则是直接检测抗原特异性CTL的有效而特异的方法，成为目前研究T细胞免疫应答的关键技术。

Advance in MHC-peptide tetramer technology

MHC-肽 四 聚 体技术研究进展

All these results indicated that high quality of recombinant protein of BF2-BSP was obtained which will be contributed to the construction of chicken MHC peptide tetramer .

实验获得了高纯度的在体外构建鸡MHC-肽 四 聚 体所需的重组蛋白BF2-BSP。

Flow cytometry analysis indicated that the tetramer could bind to specific CTL from HLA-A11 + donor .

流式细胞仪分析显示所制备的 四 聚 体可与 HLA-A11+供者的抗原特异性CTL结合。

Specific CTL responses were demonstrated by H 2K b OVA tetramer staining and cytotoxicity assay . Extracelluar IFN γ was also quantitatively analyzed .

应用 H2Kb 四 聚 体对特异性CTL检测结果与经典的细胞毒试验一致，同时对胞外IFNγ进行了定量检测。