Purification of Tetanus Toxin and Formation of Ion Channels in a Lipid Bilayer

破伤风 毒素的提纯及形成离子通道的特性

Study on gene cloning expression and immunogenicity of tetanus toxin fragment C

破伤风 毒素C片段基因克隆、重组表达及免疫原性研究

First most of impurity in the tetanus culture medium can be eliminated with HIC on Phenyl Sepharose then tetanus toxin can be further purified by the ion-exchange chromatography ( IEX ) on DEAE Sephadex .

破伤风 毒素培养滤液经PhenylSepharose疏水层析除去大部分杂质，再经DEAESephadex离子交换层析进一步纯化。

Objective To amplify clone and sequence the whole gene fragment of tetanus toxin and provide a basis for the application of it .

目的对 破伤风 毒素全基因片段扩增、克隆和测序，以便有效利用。

The protein antigen was proved to be tetanus toxin fragment C specific antigen by Western blotting .

免疫印迹实验证实， 重组蛋白抗原是 破伤风C片段抗原。

Study on purification of tetanus toxin by chromatography procedures

层析法纯化 破伤风 毒素的试验研究

The immunization with 4 doses ( 1 μ g for each ) of the expressed tetanus toxin fragment C induced sufficient antibody to protect animals from challenge with tetanus toxin .

1μgTTC经4次免疫能诱导机体产生足够的抗体，保护动物免受 破伤风毒素的攻击。

Neutralizing Activity and Preventive and Curative Effects of Human McAb to Tetanus Toxin

人源抗 破伤风 毒素单克隆抗体的中和活性和预防治疗作用

The good correlation was also observed between the tetanus antitoxin levels ( standard anti-TT horse anti-TT serum and guinea-pigs serum immunized with DTP ) detected by toxin binding inhibition ELISA and TNT .

ELISA 毒素结合抑制法测得的标抗、马抗TT血清、 DTP免疫的豚鼠血清的抗毒素水平与中和毒素试验所得结果之间也有很好的相关性。

Tetanus is a severe spastic disease caused by tetanus toxin which can be prevented by immunization with tetanus toxoid .

破伤风是由 破伤风 毒素引起的一种严重的痉挛性疾病，有效的预防方法是接种破伤风类毒素疫苗。

The toxic effects of tetanus toxin and its fragment AB on goldfish and mice were de-scribed .

作者研究了 破伤风 毒素及其AB片段对金鱼和小鼠的毒力，发现16ng的 毒素即可使金鱼麻痹和死亡；

To obtain high purity of tetanus toxin purification of tetanus toxin was carried out by chromatography procedures .

为了获得高纯度的 破伤风 毒素，用疏水层析和离子交换层析纯化破伤风毒素。

Using tetanus toxin immunofluorescence to identify nerve cells in culture

用 破伤风 毒素免疫荧光法显示体外培养的神经细胞

Experiments of Microporous Tangential flow Filtration in Clarification of Diphtheria and Tetanus Culture Suspension and Ultrafiltration in Concertration of Toxin Fluid

应用TFF微孔过滤澄清白喉、 破伤风 毒素培养液及超滤浓缩的试验

The tetanus antitoxin levels of guinea pigs immunized with TT DT and DTP were detected by indirect ELISA and compared with those by toxin neutralization test ( TNT ) . The result showed good correlation between them .

用ELISA间接法测得的TT，DT.DTP免疫豚鼠后的 破伤风 毒素水平，与用中和试验所得结果之间有很好的相关性。

Amplification Cloning and Sequencing of Whole Gene Fragment of Tetanus Toxin

破伤风 毒素全基因片段PCR扩增、克隆和序列分析

Cohclusions Sanitary delivering in the poor mountain area needs to be popularized further the inoculation rate of the tetanus toxin of women of child-bearing age should be improved ;

结论需进一步在贫困山区推广清洁接生，提高育龄妇女 破伤风 类毒素的接种率；

Toxic Effects of Tetanus Toxin and Fragment AB on Goldfish

破伤风 毒素及其AB片段对金鱼的毒力

Cloning and Expression of Tetanus Toxin Fragment C in E.coli

破伤风 毒素C部分的克隆及在大肠杆菌中的表达

Tetanus is not transmitted person to person but is caused by a toxin released by the anaerobic tetanus bacillus Clostridium tetani .

破伤风的传播途径不是从人到人，而是由厌氧性破伤风杆菌释放的 有毒物质造成。

Purification of Tetanus Toxin and Its Fragments

破伤风 毒素及其片段的纯化

Tetanus toxin and FITC labelled horse anti-tetanus toxoid serum were applied to show the plasma membrane of cultivated chicken embryonic cerebral cortical cells .

用 破伤风 毒素和异硫氰酸荧光素标记马抗破伤风血清可以特异地显示体外培养的神经细胞。

We obtained a high purity of tetanus toxin in 5 consecutive batches by using the combined chromatography procedures .

用此方法，连续纯化五批 毒素，均获得高纯度的 破伤风 毒素。试验 证明破伤风 毒素 经 疏水层析和 离子 交换层析可得到有效纯化。

Study on the Immune Regulation of Monoclonal Anti-idiotype Antibody ( Ab _2 β) Against Anti-sporozoite in P. yoelii Infected Mice Toxic Effects of Tetanus Toxin and Fragment AB on Goldfish

β型抗独特型单克隆抗体在抗疟免疫中的调节作用 破伤风 毒素及其AB片段对金鱼的毒力