Isolation of Pure Living Utricular Sensory Epithelial Cell by Use of Thermolysin

应用 嗜 热 菌 蛋白酶 快速分离活性纯椭圆囊感觉上皮细胞及其意义

This tripeptide is a fragment of cholecystokinin C-terminal octapeptide CCK-8 . Starting with Phac-Met-OCam the target tripeptide derivative was successfully synthesized with three immobilized enzymes α - chymotrypsin / Celite-545 papain / VA-Epoxy and thermolysin / Celite-545 by three reaction steps in reasonable yields .

以PhacMetOCam为起始原料，采用三种固定化酶&α糜蛋白酶/Celite545、木瓜蛋白酶/VAEpoxy、 嗜 热 菌 蛋白酶/Celite545，经过三步酶促反应得到目标三肽化合物。

Conclusion : GER isolation can be successfully performed by use of thermolysin combined with microdissection . This method may provide a good technique to establish the GER cell lines or perform series of gene transfection experiments .

结论：利用 酶 消化 和 机械解剖相结合的方法可以分离 出 纯粹的有 活性 的 GER，为进一步建立GER细胞系，进行各种基因转染试验以及 耳蜗内的 各种基础实验建立细胞模型提供有效的实验手段。

The Nitro Group as the Zinc-Binding Group Dipeptide Thermolysin Inhibitors

硝基作为锌离子结合基团的二 肽类 嗜 热 菌 蛋白酶抑制剂

Simultaneous Synthesis of Aspartame and Optical Resolution of DL-Phe-OMe by Thermolysin

用 嗜 热 菌 蛋白酶催化合成天冬甜精并同时进行DL-苯丙氨酸甲酯的光学拆分