Methods : Hematopoietic progenitor / stem cell culture and Trypan-blue detect cell viability .

方法： 采用 集 落培养及细胞活性计数方法 研究 不同 条件 下造血干 祖细胞 回收率。

Cells in logarithmic growth pHase were placed in refrigerator for gradient cooling and then were preserved in liquid Nitrogen ( - 196 ℃) for 2 ~ 6 months the viability of cells was determined by trypan-blue dye cell morpHology were also observed .

选取处于对数生长期的培养细胞梯度降温后置入液氮（-196℃）中保存4～6个月，进行细胞 复苏 培养，以 2% 台 盼 蓝确定细胞活力，并观察 复苏 的 成 纤维细胞形态学状况。

Under the microscope the inhibition effects of T-lymphoma cell and Hela cell treated by the compound were observed by TRYPAN-Blue assay .

在倒置显微镜 下， 应用 台 盼 兰 染色法观察 蟾酥氯仿 提取物对T淋巴瘤细胞及Hela细胞的生长抑制作用。

The proliferation of lymphocytes was tested with trypan-blue stain .

台 盼 蓝 拒 染法测定淋巴细胞增殖率；

Fuel cells METHODS : The cytotoxic effect of anti-EB virus delicious on B lymphocytes from umbilical cord blood was assayed by using the trypan-blue trophochrome method .

方法：用 台 盼 蓝拒染法检测抗EB病毒口服液对脐带血B淋巴细胞的细胞毒作用；

Methods Growing inhibitory test was used in vitro living cells were counted by trypan-blue staining method and calculating inhibitory percentage .

方法采用体外生长抑制实验， 台 盼 蓝 排染法计数活细胞，计算抑制百分率；