RESULTS : ① The mouse-model of Hp infecting gastric membrane : the gastric membrane of mice at 4 and 8 weeks after infection were positive in smear-microscopy urease test and Warthin-Starry stain . The infection rate of Hp was 100 % .

结果：①昆明系小鼠胃黏膜幽门螺杆菌感染模型：实验小鼠在感染4和8周后，胃黏膜涂片镜检、 尿素酶 试验及组织病理学Warthin-Starry染色检查均阳性，幽门螺杆菌感染率100%。

We have performed a prospective study on 153 consecutive symptomatic children undergoing gastroscopy using methods of histology serology urease test for Helicobacter pylori ( HP ) infection . The data of symptomatic adults and healthy children were used as controls .

我们对153例因胃肠道症状接受胃镜检查的连续患儿进行前瞻性活检组织学、 尿素酶 试验及血清学检查，探讨幽门螺杆菌（HP）感染和上胃肠道疾病关系，并和成人及健康儿童对照。

Membrane lauryl sulphate method with in situ urease test for Escherichia coli

膜法脱月桂硫酸盐法附以在原地 测试 尿素？内含的大肠杆菌

Methods The diagnosis of chronic gastritis accords with updated Sydney classification system rapid urease test histopathological staining method and 13 C breath test were used in detection of HP .

方法慢性胃炎诊断标准参照最新悉尼分类系统，幽门螺杆菌检测采用快速 尿素酶 试验、病理组织学染色及13C呼气试验。

Hp was detected by rapid urease test and Warthin starry staining .

以快速 尿素酶 试验和Warthinstarry法检测组织中的Hp；

It is supposed that the rapid urease test in combination with direct smear examination or the W-S silver method could reveal the positive rate more accurately .

作者认为，应用 尿素酶 试验同时进行直接涂片法或W-S银染法检查，可进一步提高阳性率。

METHODS Hp in gastric fluid and dental plaque was detected with fluorescent antibody method bacterial culture urease test and Hp diagnosis card at the same time in 62 cases with gastric and duodenal disease . The gather of gastric fluid applied the capsule method .

方法应用荧光抗体方法、细菌培养、 尿素酶 试验、Hp诊断卡4种方法对62例胃、十二指肠疾病患者胃液、口腔牙菌斑进行Hp检测，胃液采集采用胶囊采样法。

Methods Hp was examined in 18 patients with gastric carcinoma 36 patients with chronic gastritis and 15 controls by rapid urease test and Warthin Starry method .

方法对18例胃癌、36例慢性胃炎和15例对照组，应用快速 尿素酶 试验和Warthinstarry法检测Hp；

Methods : Rapid urease test and immunohistochemical method were used to detect the HP infection and expression of p21 and p53 in the biopsy tissues from 105 cases of gastric mucous lesion of different degrees .

方法：采用快速 尿素酶 法及免疫组化方法，检测105例不同程度胃粘膜病变活检组织中HP感染，p21、p53蛋白的表达。

Rapid urease test and serum H.pylori antibodies with ELISA or Warthin-Starry silver stains were used for H.pylori diagnosis .

pylori的诊断采用快速 尿素酶 试验和ELISA法血清H.pylori抗体或病理Warthin-Starry银染色，两项阳性诊断为H。

Objective : To evaluate the effect of rapid urease test ( RUT ) and ~ ( 14 ) C-urea breath test ( 14C-UBT ) in detecting Helicobacter pylori .

目的：评估14C-尿素呼气试验（14C-UBT）和快速 尿素酶 试验（RUT）检测幽门螺杆菌（Hp）的临床价值。

Hp infection was diagnosed by rapid urease test with gastric mucosa 13C expiration test and serum Hp-IgG test by ELISA .

采用胃黏膜快速 尿素酶 实验、Hp-13C呼气试验和血清Hp-IgGELISA检测幽门螺旋杆菌。

Biochemical identification showed that the bacteria were positive for repid urease test oxidase test peroxidase experiment and nitrate reductase test .

生化鉴定示细菌 尿素酶 实验阳性，氧化酶、过氧化物酶实验阳性，硝酸还原酶阴性。

Comparison of biopsy urease test culture and histological examination in the diagnosis of Campylobacter pylori infection

快速 尿素酶 试验与细菌培养及组织学检查对诊断幽门弯曲菌感染的比较研究

H.pylori was determined with the rapid urease test Warthin-Starry method or 14C urease breath test ;

采用Warthin-Starry染色、快速 尿素酶 试验或C14尿素呼气试验检测HP的感染情况；

Methods Hp was detected by using rapid urease test and Warthin-Starry silver stain . Inducible nitric oxide synthase ( iNOS ) was detected by using immunohistochemistry .

方法采用快速 尿素酶 试验及Warthin-Starry银染色法检测入选病例的Hp，用免疫组织化学染色法检测iNOS。

As a golden criterion with culture urease test had a sensitivity of 96.51 % and a specificity of 96 % and EIA had a sensitivity of 95.35 % and a specificity of 86 % .

以细菌培养为金标准， 尿素酶 试验的灵敏性和特异性分别为96.51%和96%，EIA法为95.35%和86%。

The sensitivity and specificity of HP infection was 88.1 % and 85.7 % respectively in urease test ;

尿素酶 试验的敏感性、特异性为88.1%、85.7%；

Methods : Urease test and PCR were used .

方法：采用 脲酶 实验及PCR方法检测 幽门 螺杆 菌。

PCR Method Compared with Rapid Urease Test to Detect Helicobacter Pylori

PCR法及快速 尿素酶 试验检测幽门螺杆菌感染的比较

Scale standard of experiment in vivo included three items : Warthin-Starry stain Hematoxylin-eosin stain and urease test with 15 points in each item and there were 45 points in all .

体内实验评分标准：共包括Warthin-Starry染色、苏木精-伊红染色、 尿素酶 试验三大项，每项15分，共45分。

Hp infection was identified by rapid urease test and histology .

Hp感染采用快速 尿素酶 试验和组织学检查来判定。

Results The positive rate is 76.6 % for rapid urease test 71 % for bacterial culture 48.1 % for direct staining and 78.5 % for PCR .

结果4种方法的检出阳性率分别为：快速 尿素酶 试验76.6%，分离培养71%，直接涂片48.1%，PCR78.5%。

Columbia blood agar was used as selective medium to isolate H. pylori . Gram-staining microscopy urease test and Oxidase test were applied to identify the isolated bacteria .

采用选择性哥伦比亚血琼脂为分离培养基，通过革兰染色镜检、 尿素酶 试验和氧化酶试验对分离的幽门螺杆菌进行鉴定。

After four-week treatment they were examined through gastroscope histology biopsy diagnosis and rapid urease test .

疗程结束后4周复查胃镜，胃黏膜活检及快速 尿素酶 试验。

All mice were killed after 4 weeks since last intragastric administration . Hp was detected by rapid urease test Giemsa dying and bacterial culture and histopathologic changes in the gastric mucosa of mice were determined by H-E staining .

最后一次灌胃4周后处死小鼠，HE染色判断小鼠胃黏膜组织学损伤，并用快速 尿素酶 试验、Giemsa染色和细菌培养进行Hp的检测。