Check the vaginal plug of females every morning ; and the day that vaginal plug was checked was counted as the day 0 of pregnancy .

每日晨检查雌 鼠 阴道脱 栓情况，检查到阴栓的当天计为 雌 鼠妊娠的第0天。

Method : ( 1 ) Preparation of animal model : The grown-up health SD rats mated 1:1 and observed vaginal plug at 8:00 in the morning The day that vaginal plug was found was recorded as gestational age 0 day .

方法：（1）动物模型制备：成年健康雌雄大鼠1：1合笼配种，每日上午8：00观察配种结果，发现 阴 栓记为雌鼠怀孕0天。

Female and male rats at the ovulation period were put in the same cage . Seen vaginal plug was set as 0.5 day after coitus .

雌性SD大鼠35只，每日做 阴道细胞涂片，排卵期雌雄合笼，以出现精 栓计为受孕第0.5天。

See vaginal smears of keratinocytes and the vaginal plug to make the day of pregnancy .

观察阴道脱落细胞涂片及 阴 栓，来确定妊娠日。