Axon patch clamp amplifier leak subtraction could subtract the leak current produced by single depolarizing pulse but not the leak current produced by a series of different step depolarizing pulses .

u653eu5927u5668u6f0fu51cfu529fu80fdu53efu6f0f u51cfu5355u4e00u53bbu6781u5316 u7535u538bu5e45u5ea6u6240u8bf1u53d1u7684u6f0fu7535u6d41uff0cu4f46u4e0du80fdu540cu65f6u5bf9u4e0du540cu7535u538bu5e45u5ea6u7cfbu5217u53bbu6781u5316u6240u4ea7u751fu7684u7a33u6001u6f0fu7535u6d41u8fdbu884cu8ffdu8e2au6f0f u51cfuff1b