Isolation of the hydrolysates and the molecular weight of the peptide were measured by Sephadex G-15 . The results showed that there were five enzymes ( trypsase papain pepsin subtilisin flavourzyme ) that can hydrolyze silver carp head into hydrolysates .
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Sequence analysis showed that members of Peptidases_S8family were highly similarity . The reported cuticle-degrading proteases are mainly belong to the PCSK9_ProteinaseK_like subfamily and Subtilisin_subset subfamily are important fungal virulence factors .
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Soybean protein isolate ( SPI ) was enzymatically modified by subtilisin . Some functional properties of modified SPI were improved .
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The mechanism of the aggregation of the peptides derived from SPI induced by subtilisin protease is also discussed in this paper in terms of the molecular structure of protein .
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Site 156 and 165 Mutation of Subtilisin E
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SPI hydrolysates induced by several commercial proteinases ( subtilisin protease chymotrypsin trypsin papain and Protex ) were analyzed by the SDS-PAGE .
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Thermodynamic Study on the Thermal Denaturation of Subtilisin
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As the members of the serine protease family acetylcholinesterase u03b1 - chymotrypsin and subtilisin had the similar structural character in active sites . All of them had the catalytic triad composed of serine histidine and asparagine ( or glutamic acid ) .
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Improvement of Antioxidation Property of Subtilisin E by Protein Engineering Method
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Extraction and Identification of Barley u03b1 - Amylase / Subtilisin Inhibitor
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Study on Protease and Cloning of Subtilisin Gene From Bacillus
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The soluble products in the hydrolysate of the complex enzyme had more amino acid scores and higher delicious amino acids content than that of bacillus subtilisin while the bitterness was on the contrary .
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The shark meat was hydrolyzed with the enzyme hydrolytic technique in which subtilisin and papain were added simultaneously .
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Many proteins belonging to the subtilisin class have this phenomenon .
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Protein Engineering on Subtilisin E
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n.枯草杆菌蛋白酶