Although the tannase treatment does give a certain amount of solubilisation of the tea cream constituents the extract is still turbid at 5 c.

尽管 丹宁 酶的处理确能溶解一部分茶膏成分，但在5°C时，这种处理过的茶浸提物仍会浑浊。

Mutation Breeding of High Tannase Activity Strains from Penicillum and Enzymatic Formation of Gallic Acid and Quinic Acid

青霉高 单 宁 酶活性菌株选育及酶法转化没食子酸及奎尼酸研究

Study on Tannase Produced by Aspergillus Niger and Propyl Gallate Biosynthesised by Immobilized Aspergillus Niger Cells in Organic Phase ; The ellagic acid has been prepared by oxidation of the leaching solution of Chinese gallnut containing tannic acid .

黑曲霉产 单 宁 酶的研究和有机相中固定化黑曲霉细胞生物合成没食子酸丙酯的初探以五倍子为原料浸取单宁酸，由单宁酸氧化制备鞣花酸。

Adopting alginate sodium as carrier and using the method of crosslinking-embedding-crosslinking Tannase and β - glucosidase were co-immobilized in this study . The activity recoveries of tannase and β - glucosidase could reach 67.3 % and 46.0 % respectively .

以海藻酸钠为载体，采用交联-包埋-交联的方法共固定化了 单 宁 酶和β-葡萄糖苷酶，2种酶的活力回收率分别为67·3%和46·0%。

Studies on the Co-immobilization of Tannase and β - glucosidase and Its Application in the Processing of Tea Beverage

单宁酶、β-葡萄糖苷酶的共固定化及其在茶饮料加工中的应用研究

In a preferred embodiment of the present invention the tannase is recycled and in that CASE higher initial levels of tannase are used .

在本发明的所选用的具体 操作中， 丹宁 酶被循环使用，因而在一开始要使用高剂量的丹宁酶。

The effects of different factors on producing tannase have been studied such as different carbon sources carbon source ratio different nitrogen sources nitrogen source ratio and initial pH value .

研究不同碳源、氮源种类和比例及不同起始pH值等因素对 黑 曲霉生产 单 宁 酶的影响；

The results showed that there was significant difference between tannase in RM and in bulk water .

结果表明： 反胶束 体系与水相中的 单 宁 酶，其 光学 行为存在很大差别。

When co-immobilized under the optimum conditions the activity recoveries of tannase and β - glucosidase could reach 67.3 % and 46.0 % respectively .

采用最优共固定化条件可使 单 宁 酶和β-葡萄糖苷酶的活力回收率分别达67.3%和46.0%。

To incubate tannase in 1 mM β - mercaptoethanol bromine and ammonia acrylic acid for 30 min residual activity was 41.2 % 54.6 % and 66 % respectively .

单 宁 酶在1mMβ-巯基乙醇、溴乙酸和氨腈三种抑制剂中培育30min后，剩余酶活分别为41.2%、54.6%和66%。

Tannase hydrolyses the ester bonds and depside bonds in gallic acid tannins to release gallic acid and glucose .

单 宁 酶可水解没食子酸单宁中的酯键和缩酚羧键，生成没食子酸和葡萄糖。

It was found that the strain have not only activity of tannase but also activity of polyphenol oxidase ( PPO ) .

结果表明这株菌不仅具有 单 宁 酶的活性，而且具有多酚氧化酶（PPO）的活性。

Tannase not only catalyze hydrolysis reaction but also catalyze esterification . For example it can transfer ester bone of tannic acid to alcohols to synthesis gallic acid esters directly .

单 宁 酶不仅能催化水解反应，还能催化酯化反应，例如它可将单宁酸中酯键转移给醇类直接合成没食子酸酯类。

Optimization of conditions of tannase production with Gallnut by unsterile fermentation using response surface methodology

响应曲面法优化五倍子生料发酵产 单 宁 酶的条件

Tannase ( TA ) was immobilized by calcium alginate gel . 60 6 % of TA was recovered .

采用海藻酸钙凝胶对 单 宁酶进行固定化， 酶 活力回收率高达 60.6%。

The obtained hot tea extract is then cooled to a temperature which make it suitable for the tannase treatment .

把获得的这种热水提取物冷却到一个适合进行 丹宁 酶处理的温度。

Tannase is widely used in beverage food leather chemical and pharmic industry .

单 宁 酶在饮料，食品，制革，化工，药业等方面都有着广泛的应用。

The Effects of co-immobilized Tannase and β - glucosidase on the Aroma-increasing and Haziness Removing in Tea Beverage

共固定化 单 宁 酶和β-葡萄糖苷酶对茶饮料增香和除混效果的研究

Compared with their free enzymes the stabilities to thermal acid and alkali and chemical reagent as well as storing stability of co-immobilized tannase and β - glucosidase were markedly enhanced .

共固定化后两种酶对热、 酸碱、化学试剂的稳定性和贮藏稳定性都有显著提高；

Study on Gallic Acid Preparation by Using Immobilized Tannase from Aspergillus niger

利用固定化黑曲霉 单 宁 酶制备没食子酸的研究

Mutation breeding of high tannase activity strains from Aspergillus niger

黑曲霉 单 宁 酶高活性菌株的诱变选育

Optimization of the production of tannase by Aspergillus niger solid-state fermentation using gall nut

黑曲霉利用五倍子生料固体发酵产 单 宁 酶优化发酵条件研究

An enzyme level ranging from about 0.5 to 500 or more tannase units ( determined as hereinbefore described ) per gram dry weight of tea may be used .

酶的用量为每克干茶叶用0.5-500或更多的 丹宁 酶单位（用如前所述的方法测定）。

RM was propitious to tannase stability and it was enhanced with the increase of alkyl alcohol moieties carbon length .

反胶束 体系有利于 单 宁 酶的稳定，脂肪醇作为反应底物，其碳链的增长有利于 单 宁 酶在反胶束中的稳定性。

The method used for the determination of tannase activity of enzyme preparation is a modification of the method described by R.

用于测定酶制剂中 丹宁 酶活性的方法，是一个对R。

Study on clarification of green tea extract with immobilized tannase by uniform design

均匀设计法在固定化 单 宁 酶澄清绿茶 汤 工艺中的应用

Tannase was dissolved in RM with inserting RM membrane or adhering to RM .

单 宁 酶是以嵌入反胶 束膜或与 反胶束 内膜 接触的方式增溶的。

Primary Study of Tannase Hydrolysis Advancing the Quality of Green Tea Infusion

单 宁 酶水解提高绿茶茶汤品质的初步研究

The tea extract which has been treated with tannase is subsequently subjected to an ultra-filtration treatment .

随后，用 丹宁 酶处理过的茶叶提取物要经过超过滤处理。