The Transcriptive Regulation of Cultured Cells in Saccharometabolic Abnormality

培养细胞对糖代谢异常环境的基因 应答研究

Recent research advances in multiple bioinformatics ( computational molecular biology ) and high-throughout experimental technologies have made the reconstruction of transcriptive regulatory networks possible .

最近多种生物信息学（计算分子生物学）工具和高通量实验技术的发展，使得重建 复杂 的 基因调控网络成为可能。

Objective Hypoxia-inducible factor ( HIF ) - 1 α is a transcriptive factor that regulates genes involved in metabolism angiogenesis and metastasis .

目的缺氧诱导因子1α（HIF-1α）是 存在 于 人和哺乳动物 细胞内的缺氧 应答调节因子，对缺氧 环境 下维持肿瘤细胞能量代谢、新血管形成及转移过程起重要作用。

Objective : To explore the glycobiology-related pathogenesis of diabetic retinopathy by studying the transcriptive regulation of stringently cultured cells responsive to the changes of basic components involved in the process of glucose metabolism .

目的：通过研究体外培养细胞在严格控制的条件 下对微环境内、外糖代谢基本物质含量变化的 基因 应答，深入探索发生在糖尿病视网膜病变中的糖生物学调控机制。

The semi - quantitative reverse transcriptive PCR was performed to detect the expression of TGF - β 1 CTGF and FN mRNA .

RT-PCR半定量 分析 HK2 细胞中TGF-β1、CTGF和FnmRNA的表达水平；

Purpose : Tissue microarray investigates the expression of hypoxia-inducible factor-1 α( HIF-1 α) and its relationship to multi-drug resistance protein ( MRP ) in bladder cancer . HIF-1 α is a transcriptive factor that regulates genes involved in metabolism angiogenesis proliferation and apoptosis of carcinoma .

目的：利用组织芯片研究缺氧 诱导因子（HIF1α）和多药耐药相关蛋白（MRP）在膀胱癌中的表达及其 相互 间的关系。