Study on the spectrophotometric determination of aminoglycoside antibiotics with trypan red

曲 利 本 红分光光度法测定氨基糖苷类抗生素

Isolation purification culture and identification of rat Sertoli cells in vitro : Take 18 to 21 day-old male SD rat testis culture primary Sertoli cells in vitro identify cell viability by trypan blue and identify cells purity by oil red O.2 .

方法1.体外分离、纯化、培养和鉴定大鼠睾丸支持细胞：取18~21日龄雄性SD大鼠的睾丸组织，体外原代培养睾丸支持细胞， 苔 盼蓝鉴定细胞活力，油 红O鉴定细胞纯度。

In acid solution amikacin sulfate ( AMK ) react with tiger red ( TIR ) or trypan red ( TRR ) to form an ion-association complex .

在酸性条件下，硫酸阿米卡星（AMK）与虎红（TIR）、 曲 利 本 红（TRR）均可反应生成离子缔合物。

Conclusion It is the same effect to detect the activity of the cells between trypan blue and neutral red .

结论 锥 虫蓝和中性 红 染色，都能较好地检测细胞的活性。

Spectrophotometric determination of amikacin sulfate with tiger red and trypan red

虎红、 曲 利 本 红-阿米卡星的光度法研究

Methods Mix-cultured conical neurons of SD rats were subjected to mechanical scratch injury and the degree of injury were assessed by cell count with trypan blue staining and by lactate dehydrogenase ( LDH ) test . The intact cells were confirmed with neutral red counter staining .

方法采用机械划痕创伤性损伤培养大鼠皮层神经元，根据培养细胞 锥 虫篮活力计数和培养上清乳酸脱氢酶（LDH）检测评估损伤程度，用中性 红复染确定未损伤细胞。

Staining the separated cells by trypan blue and neutral red .

锥 虫蓝和中性 红染色检测分离细胞的活性。