The results showed : zona pellucida begin arise while follicules with 2 or more layers of granulosa cells .

结果显示：当 卵母细胞由2层 卵泡细胞包围时，开始形成透明带；

We found that the two Mab had cross-reaction with zona pellucida and ovum cytoplasm and no cross-reaction with membrana granulosa and theca folliculi .

发现它们除对人 卵透明带有交叉反应外，还在卵细胞质内见有反应 颗粒，而对 卵泡 颗粒 层及卵泡膜则无反应。