A high activity and long life immobilized uricase has been prepared . It was applied to determine uric acid in serum and urine using the flow injection chemiluminescence method .

本文制备了高活性和长寿命固定化 尿酸 酶，并用流动注射化学发光分析在线测定了人血清和尿中尿酸含量。

Screening of Uricase Producing Strains Cloning and Expression of Uricase Gene

尿酸 酶产生菌的筛选、基因克隆及表达

The human uricase gene is extracted by this laboratory from human liver organization and preserved in BL21 ( DE3 ) expression strain .

人 尿酸 酶基因是由本实验室从人肝脏组织中提取的，保存在大肠杆菌BL21（DE3）菌株中。

Exploratory Development of Uricase Inhibitor for Replicating Acute Animal Model of Hyperuricemia on Rat

尿酸 酶抑制剂在大鼠急性高尿酸血症模型复制中的应用

Bioelectrochemical Activities of the Polypyrrole Uricase Electrode

聚吡咯 尿酸 酶电极的生物电化学活性

Uricase ( UOX ) is a key enzyme in the metabolic pathway of purine which decides the final breakdown product of purine in living organism .

尿酸 氧化酶是生物体内嘌呤代谢途径中最后一个关键酶，决定了嘌呤代谢的最终产物。

Both the activity and stability of the finalized uricases were improved compared to the original uricase .

最终筛选到的人犬嵌合尿酸 酶与初始犬源 尿酸 酶相比，活性和稳定性均有显著提高。

Study on the characteristics showed that : the optimum pH for uricase reaction was 8.5 and the optimal temperature was 30 ° C.

研究 尿酸 氧化酶 的 理 化特性得知：该 酶的最适反应pH为8.5，其最适作用温度为30℃；

Poly-2-aminopyridine electrode is more stable than polyaniline-uricase electrode because the latter is affected by uricase activity .

聚-2-氨基吡啶修饰电极比聚苯胺 尿酸 酶电极更稳定，因为聚苯胺 尿酸 酶电极受 尿酸 酶活性的影响。聚-2-氨基吡啶对尿酸的催化反应活化能Ea为23kJ。

Study on Physicochemical Properties and Pharmacodynamic Action of Recombinant Uricase

重组 尿酸 酶 部分理化性质及其 初步药效学研究

The necessity and present conditions of uricase biosensor are introduced .

文章简要介绍了 尿酸 酶生物传感器研究的必要性及国内外研究现状；

Inhibitory effects of xanthine on uricase analyzed by a linear kinetic method

用线性动力学方法考察黄嘌呤对 尿酸 酶的抑制作用

Uricase EC 1.7.3.3 w_1306 is an enzyme involved in the purine degradation pathway and catalyzes the hydrolysis of uric acid to the more water-soluble allantoin .

尿酸 酶EC1.7.3.3 w_1331是嘌呤代谢过程中的一种酶，它可将尿酸水解为更易溶于水的尿囊素。

Study on Purification and Characterization of Uricase from Candida utilis

产朊圆酵母 尿酸 酶的分离纯化及其 部分性质研究

Characterization of an Extracellular Uricase from Bacillus fastidious and Its Application in Direct Kinetic Assay of Serum Uric Acid

苛求芽孢杆菌胞外 尿酸 酶的表征及其在血清尿酸测定中的应用

Expression of Recombinant Uricase in E. coli JM109 ( DE3 ) Induced by Lactose

乳糖诱导重组 尿酸 酶基因在大肠杆菌中的表达

Results Hypoxanthine was administered via stomach while uricase inhibitor was injected subcutaneously by lanolin mixed with olefin which made the uric acid in serum higher than control group about 12 hours .

结果采用灌胃给予次黄嘌呤，皮下注射以羊毛脂 石 蜡油为溶剂配制的 尿酸 酶抑制剂，可使受 试 动物血尿酸值明显高于空白对照组（ P<0.01）， 且可维持12h。

METHODS : Uricase reaction curve was monitored by absorbance at 293 nm .

方法： 用 293nm吸收记录 尿酸 酶反应。

The biosensors are prepared by immobilizing glucose oxidase and uricase respectively on the surface of screen-printed carbon paste electrodes modified by carbon nanotubes while potassium ferricyanide is employed as electron transfer mediator .

在丝网印刷碳糊电极上利用吸附法固定葡萄糖氧化酶或 尿酸 酶，并用碳纳米管进行修饰，铁氰化钾作为电子传递剂，制作用于测量人体血浆中葡萄糖和 尿酸 浓度的生物传感器。

Molecular weight of the recombinant uricase subunit was 32.4 ku in SDS-PAGE .

结果：经SDS-PAGE检测重组 尿酸 酶亚基的 表 观相对分子质量为32.4k。

Most mammals have a gene that makes an enzyme called uricase that breaks down uric acid but humans lack the ability .

大多数哺乳动物都有一个叫做 尿酸酶的基因来分解尿酸，但人类缺乏这种能力。

The purified uricase migrated as a single protein band on reduced / non-reduced SDS-PAGE . The purity analyzed by HPLC is above 98 % .

纯化后的 酶经还原、非还原 SDS-PAGE分析为单一蛋白染色带，HPLC显示纯度为98%以上。

Inhibition of Bioelectrochemical Activities of Polyaniline Uricase Electrode by Theophylline

茶碱对聚苯胺 尿酸 酶电极的生物电化学活性的抑制

The study of properties for the polyaniline uricase electrode

聚苯胺 尿酸 酶电极性能的研究

This method was further used to detect UA and uricase by coupling with enzymatic reaction .

这种方法通过酶促反应进一步用于检测尿酸及 尿酸 酶。

75 % ammonium sulfate fractionation can get the most uricase activity field .

硫酸铵沉淀 尿酸 酶的最佳 浓度为75%。

Study On The Nanoparticles Strengthen Uricase Biosensor

纳米颗粒增强 尿酸 酶生物传感器的研究 设想

Uricase gene mutations of human may result in the formation of pseudogenes .

人类 尿酸酶基因因突变而成为假基因， 如果能使之复活，必然能够在 很大 程度上 降低该 酶的免疫原性。

The uric acid concentration was measured by uricase peroxidase method .

UA含量测定采用 尿酸 酶 -过氧化物酶 偶联法。