Smearing vagina mucosa cell HE staining cytology examination .

然后 阴道 粘膜 脱落 上皮细胞 涂片、HE染色、细胞学检查；

Objective In order to improve the treatment of vagina relaxation a modified colporrhaphy with the vagina mucosa intact is presented .

目的：对不损伤 阴道 粘膜的阴道紧缩手术进行部分改良，以提高手术效果。

The irritation reaction intensity of rat vaginal mucosa to mucosa disinfectants was no irritation while the vagina disinfectants causing very mild irritation of rabbit vaginal mucosa accounted for 37.5 % .

黏膜消毒剂对大鼠阴道黏膜刺激反应强度均为无刺激性，对家兔阴道 黏膜有 极轻刺激性者占37.5%；

Methods : Vaginal mucosa cells could be harvested from human vagina mucosa treated with trypsin-collagenase digesting .

方法：用胰酶、胶原酶混合消化法获得 阴道 黏膜细胞。以丝裂酶素C处理的 小鼠成纤维细胞作滋养层细胞。

Methods In order to make vagina mucosa and muscular layer shrinkage and swelling and to deflate vagina the absorbable ligature or 3-0 suture silk thread were used for the patients with vaginal relaxation to circular suture and tie continuously at the submucous muscular layer .

方法应用3-0丝线或可吸收线，对自觉阴道松弛的患者行黏膜下肌层内连续缝合，环状收缩打结，使 阴道 黏膜及肌层皱缩隆起，缩小阴道内腔。

Conclusion The modified colporrhaphy with vagina mucosa intact is a safe and efficacy technique .

结论：经过改良的保全 阴道 粘膜的阴道紧缩手术是一种安全有效的手术方法。

Build up the animal model of artificial vagina with human vaginal mucosa stem cells

建立人阴道 黏膜干细胞构建人工 阴道的动物模型研究

A modified colporrhaphy with the vagina mucosa intact Vulva and Vagina

保全 阴道 粘膜的阴道紧缩术

Results The acute oral LD_ 50 of iodophor disinfectant was > 5000 mg / kg ( body-weight ) the disinfectant is actually non-toxic substance . Mouse bone marrow polychromatic erythrocyte micronucleus tests was negative . The irritation index for rabbit skin and eye and rat vagina mucosa was zero .

结果该碘伏消毒液LD50大于5000mg/kg，属实际无毒，小鼠骨髓细胞微核试验为阴性，碘伏消毒液原液对家兔皮肤、眼及 阴道 黏膜刺激的刺激指数均为0，均属无刺激性；

The change of vagina mucosa cell smear : The content of estrogen in model group was significantly lower than that of sham operated group .

大鼠 阴道 脱落细胞涂片的变化：模型组较假手术组雌激素水平显著低落（p＜0.05）；