OBJECTIVE To identify the active components in Venenum Bufonis by high performance liquid chromatography ( HPLC ) - electrospray – time of flight mass spectrometry ( ESI-TOF-MS ) .

目的采用高分辨电喷雾飞行时间质谱（ESI-TOF-MS）联用技术，对片 蟾酥中的部分成分进行了初步鉴定，探讨HPLC-ESI-TOF-MS联用技术作为蟾酥质量控制方法的 可行性。

Determination of the Quantity of Venenum bufonis in Feng You Jing by TLC Dual Wavelength Scanning Method

双波长薄层扫描法测定 风油精中华蟾毒精的含量

Analysis of Flavonols Compounds in Flos Abelmoschus Manihot . by High Performance Liquid Chromatography-Electrospray Ionization / Quadrupole-Time of Flight-Mass / Mass Spectrometry Rapid Quantification and Identification of Bioactive Components in Venenum Bufonis by HPLC-DAD / ESI-TOF-MS

高效液相色谱-电喷雾飞行时间质谱分析 蟾酥有效成分

Methods : Extracted Venenum Bufonis remains was determined by physical and chemical test and TLC .

方法：采用理化鉴别和薄层色谱鉴别法对提取 后的蟾酥残渣进行检查。

Active composition pharmacological action and Clinical practice of Venenum Bufonis was discussed .

本文对 蟾酥含有的多种活性成分，药理作用和临床应用进行论述。

AIM : To observe the intervention of resibufogenin one of main components of Chinese herb venenum bufonis on the growth of human gastric cancer BGC-823 cell line cell cultured in vitro and the relationship with cytochrome C and Caspase-3 activation .

目的：观察中药 蟾酥主要成分之一&脂蟾毒配基对体外培养人胃癌BGC-823细胞系细胞生长的干预及其与细胞色素C和半胱氨酸天冬氨酸蛋白酶3活化的关系。

Anti-streptococci infection activities of Venenum bufonis and its effective fraction and constituents

蟾酥抗链球菌感染的活性物质基础研究

Quality assessment of Venenum Bufonis by chemical pattern recognition

蟾酥薄层色谱数据的化学模式识别

CONCLUSION HPLC-ESI-TOF-MS is a very powerful tool for the identification of the effective components in Venenum Bufonis .

结论高效液相色谱-电喷雾飞行时间质谱 联用技术是 蟾酥质量控制的有效方法。

Methods : Using HL-60 cells as experimental object the experimental research of Chansu ( toad cake Venenum Bufonis ) the chief component of Liu Shen Wan was carried out .

方法：选择HL-60细胞为实验对象，以六神丸及其主要成分之一的 蟾酥进行该实验。

Clinical application of venenum bufonis

蟾酥的临床应用

Preparation and evaluation of long-circulating liposomes of Venenum Bufonis extractive

蟾酥提取物长循环脂质体的制备及质量评价

Research on the Active Analgesic Component of Venenum Bufonis

蟾酥的镇痛活性成分

Study on the Identifying Methods of the High or Low Quality of VENENUM BUFONIS by Using Capillary Gas Chromatography

优劣 蟾酥的毛细管气相色谱鉴别方法

Results : The optimum extraction technology of Venenum Bufonis is using 90 % and 80 % alcohol extraction .

结果： 蟾酥最佳提取工艺为采用90%、80%乙醇 依次提取。